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KMID : 0369820130430020145
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Jorunal of Korean Pharmaceutical Sciences
2013 Volume.43 No. 2 p.145 ~ p.151
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Proliferation and chondrogenic differentiation of human adipose-derived mesenchymal stem cells in sodium alginate beads with or without hyaluronic acid
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Kim Dong-Hwan
Kim Dae-Duk
Yoon In-Soo
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Abstract
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Human adipose-derived mesenchymal stem cells (AD-MSCs) have attracted much interest as an alternative to autologous chondrocytes and bone marrow-derived mesenchymal stem cells for cell-based therapy to repair cartilage defects. Sodium alginate (SA) beads have been widely known as a conventional stem cell delivery system for cartilage repair. Hyaluronic acid (HA) has been known to induce cell proliferation and chondrogenic differentiation. Herein, we prepared AD-MSCs-encapsulating SA beads with HA (SA?HA beads) and without HA (SA beads). Then, the morphology, proliferation, and chondrogenic differentiation of AD-MSCs cultured in SA?HA beads or SA beads with a conventional chondrogenic media were evaluated. There was no discernible difference in the morphology of AD-MSCs between SA?HA and SA beads. However, the proliferation (MTT optical density and DNA contents) and chondrogenic differentiation (s-GAG contents and type II collagen staining) of AD-MSCs were significantly enhanced in SA?HA beads as compared to SA beads. The present results suggest that HA can be added to SA beads-based cell delivery systems of AD-MSCs in order to improve their chondrogenesis-inducing capacity for repair of cartilage defects.
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KEYWORD
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Human adipose-derived mesenchymal stem cells, Sodium alginate beads, Hyaluronic acid Proliferation, Chondrogenic differentiation
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